International Journal of Food Nutrition and Safety
ISSN: 2165-896X (online)Search Article(s) by:
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Table of Content for Vol. 7 No. 2, 2016

Microbiological Quality of Raw Milk and Associated Health Risk in the Hyderabad Region of Pakistan
Tahmina Shah, Qurban Ali Shah, Jan Muhammad Shah, Muhammad Asif Arain, Muhammad Saeed, Farma Ali Siyal, Rab Nawaz Soomro and Sarfraz A. Brohi
      
 PP. 61 - 77
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ABSTRACT: The aim of this study is to evaluate the microbial quality and associated health risk of raw milk stored at surrounding intermediaries of Hyderabad Pakistan. The microbial quality assessment of marketed raw milk was undertaking by evaluating 120 raw milk samples from selling points i.e. at farm level, dairies and retailer level each containing (n=40) samples in Hyderabad city. All samples were evaluated for total viable (TV), coliforms and yeasts-molds counts. TV Count appeared significantly (P<0.05) high in milk stored before sale at large dairy farms compared to that of milk at small dairy farms. Total count in dairy farms milk samples (1.08x105±3.27x103cfu/ml) was comparatively lower than that of retailer shops (1.30x105±5.80x102cfu/ml) and dairies (1.18 x 105 ± 6.56 x 102 cfu/ml). Moreover, total viable count of milk samples obtained from dairy farms and dairies was non-significant but significantly lower (P<0.05) than that of retailer shops. The average coliform count of retailer shops milk samples (5.06 x 104 ± 8.86 x 103 cfu/ml) was comparatively higher (P<0.05) than that of dairies (4.59 x 104 ± 6.91 x 102 cfu/ml) and dairy farm milk samples (4.24 x 104 ± 7.60 x 103 cfu/ml), respectively. While the coliform count in all milk samples of different intermediaries was significantly different (P<0.05) from each other; Yeasts and molds count of retailer shops milk samples (2.17 x 104 ± 5.22 x 102 cfu/ml) was significantly high followed by dairies (1.94 x 104 ± 3.86 x 103 cfu/ml) and dairy farm milk samples (1.80 x 104 ± 4.42 x 103 cfu/ml). It can be concluded that the storage condition of raw milk in the study area is of poor microbiological quality. This highlights the urgent need to adapt good sanitation practices and active monitoring from production through the distribution chain to the consumer.


Simultaneous Detection of Listeria monocytogenes and Salmonella enteritidis by Multiplex PCR Method in Poultry Meat Samples
Hashem Gharedaghi, Golnaz Asaadi Tehrani, Razzagh Mahmoudi and Hossein Gholampour
      
 PP. 78 - 87
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ABSTRACT: The aim of this study was to adapt the multiplex PCR technique on the rapid and direct identification of the presence of Salmonella enteritidis and Listeria monocytogenes in poultry meat samples. Specific primers for multiplex PCR amplification of the, hlyA, actA, ttrC and sdfI genes were designed to allow simultaneous detection of Listeria monocytogenes, and Salmonella enteritidis respectively. The implementation of the standard technique using positive controls was successfully adapted. Following enrichment culturing for 20–24h at 37°C in TSBYE, the samples were subjected to for DNA extraction. Four fragments of the expected sizes were amplified in a single reaction and visualized in all of the samples inoculated with ≤ 10 CFUg-1. Results can be obtained in approximately after 30 hours. The results of our research for simultaneous detection of L. monocytogenes and S. enteritidis in poultry meat samples showed that the mPCR is able to detect Salmonella enteritidis in 4.76% and Listeria monocytogenes in 6.34 % and both of them in 1.58% of samples. The multiplex PCR is a potentially powerful technique for rapid and sensitive co-detection of both pathogens in foods and other types of samples.