International Journal of Food Nutrition and Safety
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Table of Content for Vol. 12 No. 1, 2021

Phytochemical Analysis and Antimicrobial Activity of Seed Oils of Parkia Biglobosa and Syzygium Aromaticum on Escherichia Coli and Staphyloccocus Aureus
Lami Nnamonu, Accord Solomon and Terrumun Amom Tor-Anyiin
      
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ABSTRACT: Nature-based products, including plant secondary metabolites are employed in a bid to combat bacterial resistance, which is a major cause of failure in the treatment of infectious diseases. This study was designed to test the effectiveness of oils of Parkia biglobosa and Syzygium aromaticum seeds against Staphylococcus aureus and Escherichia coli in order to ascertain suitability of the oils as food preservatives for the control of the organisms on cooked food. The study involves the antibacterial activity of the oils on cooked rice spiked with E. coli 0157:H7 and S. aureus. Oil extracted from P. biglobosa seeds was subjected to qualitative phytochemical analysis using standard methods. This revealed the presence of saponins, flavonoids, terpenoids, steroids and glycosides. Antibacterial activity of oils of P. biglobosa and S. aromaticum were carried out using agar diffusion and broth dilution methods. The test organisms were laboratory isolates of E. coli and S. aureus. Results revealed that both oils possess significant anti-E. coli and anti-S. aureus activity at various concentrations (25%, 50% and 100%). Oil of P. biglobosa seeds showed stronger anti-E.coli activity at 50% concentration and strong anti-S. aureus at 25 % concentration. Oil of S. aromaticum with bud showed strong anti-E. coli at 100 % concentration and showed no anti-S. aureus activity. The oil of S. aromaticum without bud showed strong anti-E. coli activity at 100% concentration and anti-S. aureus at 50% concentration. This study revealed medicinal value addition to the traditional uses of the oils of P. biglobosa and S. aromaticum seeds. These oils have veritable potential for treatment of common food-borne diseases caused by E. coli and S. aureus.