ABSTRACT: The presence of antibiotic-resistant enterobacteria in fresh vegetables can be considered as a silent food safety concern, contributing to dissemination of resistances in the community. Here, we have studied the presence of Enterobacteriaceae isolates in organic fresh produce and the resistance of these isolates to eleven chemotherapeutic agents. A total of 50 samples of organic fresh vegetables (carrot, tomato, arugula and lettuce) marketed in Valencia (Spain) were analyzed. More than half of the vegetable samples analyzed (27 out of 50, 54%) were positive for the presence of coliform bacteria, including samples of all types of vegetables tested (90% arugula, 70% carrot, 50% lettuce and 10% tomato samples). A total of 37 isolates belonging to the Enterobacteriaceae family were identified: Pantoea agglomerans (nine isolates), Serratia marcescens (six) and Enterobacter cloacae (five), among others. Only five isolates were susceptible to all agents tested (13%), and no resistances to streptomycin, ceftazidime, ciprofloxacin, co-trimoxazole or chloramphenicol were found. Most isolates were resistant to ampicillin (31 out of 37), and 15 isolates showed resistance to amoxicillin/clavulanic. Resistances to other agents were less frequent: nitrofurantoine (six isolates), cefotaxime (four), tetracycline (two), and gentamicin (one). Multiresistances to two (ten isolates), three (one isolate) and four agents (five isolates) were also observed. These results point out that control of organic fresh produce should be improved to avoid contamination with antibiotic-resistant bacteria, probably from human and/or animal origin, which can be considered as a serious issue concerning the quality of organic produce.