ABSTRACT: The aim of this study was to adapt the multiplex PCR technique on the rapid and direct identification of the presence of Salmonella enteritidis and Listeria monocytogenes in poultry meat samples. Specific primers for multiplex PCR amplification of the, hlyA, actA, ttrC and sdfI genes were designed to allow simultaneous detection of Listeria monocytogenes, and Salmonella enteritidis respectively. The implementation of the standard technique using positive controls was successfully adapted. Following enrichment culturing for 20–24h at 37°C in TSBYE, the samples were subjected to for DNA extraction. Four fragments of the expected sizes were amplified in a single reaction and visualized in all of the samples inoculated with ≤ 10 CFUg-1. Results can be obtained in approximately after 30 hours. The results of our research for simultaneous detection of L. monocytogenes and S. enteritidis in poultry meat samples showed that the mPCR is able to detect Salmonella enteritidis in 4.76% and Listeria monocytogenes in 6.34 % and both of them in 1.58% of samples. The multiplex PCR is a potentially powerful technique for rapid and sensitive co-detection of both pathogens in foods and other types of samples.