International Journal of Food Nutrition and Safety
ISSN: 2165-896X (online)Search Article(s) by:
Author Name:
Current Issue: Vol. 7 No. 1or Keyword in Title:
Editorial Email: ijfns@modernscientificpress.comor Keyword in Abstract:
RSS http://www.modernscientificpress.com/RSS/IJFNS_RSS.xml        

Table of Content for Vol. 7 No. 1, 2016

Rapid Identification of Salmonella Typhimurium Using invA Gene and Three Genome Regions (TSR1, TSR2 and TSR3) in Milk as a Food Model by Multiplex PCR Detection
Hossein Gholampour, Razzagh Mahmoudi, Payman Zare and Hashem Gharedaghi
      
 PP. 1 - 9
       View Full Paper        Download Full Paper

ABSTRACT: Contamination of Milk with Salmonella is a major risk factor for human health and multiple outbreaks of salmonellosis by milk contamination have been reported. To decrease the time and labor requirements of conventional detection methods, rapid and sensitive methods mainly based on PCR amplification are preferred. The aim of the present study was improvement and optimization of multiplex PCR amplification of serovar-specific genomic regions for the direct detection and serotyping of Salmonella Typhimurium in milk. Samples of previously sterilized milk were inoculated with 10 to 105 CFU/mL of S. Typhimurium LT2 and tested for multiplex PCR of 4 sequences including invA gene as the marker of Salmonella and 3 SSGRs specific for S. Typhimurium. Direct plate counting and parallel PCR with filter-purified extracted DNA were simultaneously performed to validate the results. After several tests the lowest detection limit of the method for milk samples was determined. Although the detection limit was 10 CFU/mL, the sensitivity decreased in this concentration especially for larger PCR products. Results of this study in conjunction with control evaluations proved that this method can be used routinely as a sensitive and rapid method with overall 3 hours time requirement for the detection of S. Typhimurium in milk samples.


Detection of ESBL Genes in Salmonlla enteritudis Isolated from Clinical Samples
Razzagh Mahmoudi, Kiumars Amini, Ata Kaboudari, Seyyede Faezeh Rahimi Pir Mahalleh, Babak Pakbin
      
 PP. 10 - 25
       View Full Paper        Download Full Paper

ABSTRACT: Salmonella enteric serovar enteritidis is currently the most common serovar causing salmonellosis in human. The incidence of gastrointestinal infections caused by S. enteritidis has increased during the last decade. The aim of this study was to extract ESBL genes from S. enteriditis separated from clinical samples using multiplex PCR. In this study, 29 human fecal samples of Collections microbial Azad University"s research were collected. After enrichment and isolation and DNA extraction SipB/C, CmlA/tetR, TEM, PSE-1genes by multiplex PCR were evaluated. A total of 29 clinical samples studied, 6 (68.20%) of the strains were positive for genes SipB / C, CmlA / tetR, TEM specimens were observed. Detection of S. enteritidis strains by molecular methods are very accurate and can be done quickly. Studying these genes in various other sources as well as the antibiotics profile is recommended.