ABSTRACT: Objective: To research the antibacterial effect of deionized water and ethanol-water extracts from the dried flowers used in Guangdong traditional soup on 7 kinds of familiar intestinal pathogenic bacteria. Methods: We selected 8 types of dried flowers most commonly used in making Guangdong traditional soup, including Rafflesia arnoldii, Bombax ceiba, Dolichos lablab, Pueraria lobata, Flos buddlejae, Lonicera japonica, Flos sophorae and Cordyceps militaris. Seven target bacteria were chosen from usual intestinal pathogenic agents, which included Staphylococcus aureus, Escherichia coli, Salmonella typhimurium, Shigella flexneri, Bacillus cereus, Pseudomonas aeruginosa and Staphylococcus epidermidis. Bacteriostasis rate, MIC and MBC were examined to evaluate the dried flowers’ antibacterial activity on these pathogens. Results: Our findings showed that all the samples exhibited potent antibacterial activity. Gram-positive bacteria were generally more sensitive to the tested extracts than Gram-negative ones. Staphylococcus aureus was the most sensitive, while Escherichia coli was the most resistant. The antibacterial activity of Cordyceps militaris was the strongest, and Flos sophorae and Lonicera japonica followed. At the same time, the antibacterial effect of ethanol-water extract was higher than that of deionized water extract. Conclusions: This was the first time to study the antibacterial property of principal material used in Guangdong traditional soup. These results might underline the theoretical foundation for the dried flowers related Guangdong Traditional Soup’s function of adjusting human body’s healthy condition which we thought was in relationship in some extent with mild inflammation of upper respiratory tract and tonsils. The more explicit mechanism of the antibacterial effect should be further studied.