ABSTRACT: Salmonella enteric serovar enteritidis is currently the most common serovar causing salmonellosis in human. The incidence of gastrointestinal infections caused by S. enteritidis has increased during the last decade. The aim of this study was to extract ESBL genes from S. enteriditis separated from clinical samples using multiplex PCR. In this study, 29 human fecal samples of Collections microbial Azad University"s research were collected. After enrichment and isolation and DNA extraction SipB/C, CmlA/tetR, TEM, PSE-1genes by multiplex PCR were evaluated. A total of 29 clinical samples studied, 6 (68.20%) of the strains were positive for genes SipB / C, CmlA / tetR, TEM specimens were observed. Detection of S. enteritidis strains by molecular methods are very accurate and can be done quickly. Studying these genes in various other sources as well as the antibiotics profile is recommended.