ABSTRACT: The present study was carried to find out the impact of sugar mill effluent on photosynthetic pigment content and biochemical constituents variance of Cluster Bean (Cyamopsis tetragonaloba (L) Taub). All the photosynthetic Pigment content; such as chlorophyll-a, chlorophyll-b, total chlorophyll, carotenoid content and biochemical constituents, such as, starch, amino acid, protein, reducing and non-reducing sugar content were increased at 10 % sugar mill effluent concentration of cluster bean plants. The above parameters were decreased at higher (25, 50, 75 and 100 %.) concentrations of sugar mill effluents.