ABSTRACT: Naringenin is the main active component in peach leaves and has been used for prevention of several diseases, including colitis, atherosclerosis and metabolic dysregulation. In the present study, naringenin was extracted from peach (Prunus persica) leaves by ultrasonic-assisted extraction. Several important parameters such as ethanol concentration, solid-to-liquid ratio, extraction temperature and extraction time were optimized by single-factor experiment and response surface methodology. The optimal conditions were 52.2% ethanol, the liquid-to-solid ratio was 30:1, and extraction for 18.3 min at 62.0 °C under ultrasound irradiation of 200 W. Under these conditions, the yield of naringenin reached 66.17 mg/g, significantly higher than that of conventional extraction. The results obtained are very favorable for the full utilization of peach leaves, and also indicate that ultrasonic-assisted extraction is a convenient method for extracting naringenin from plant materials.