International Journal of Modern Biology and Medicine
ISSN: 2165-0136 (online)Search Article(s) by:
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Table of Content for Vol. 6 No. 2, 2015

Response Surface Methodology for Ultrasound-Assisted Extraction of Naringenin from Peach (Prunus persica) Leaves
Ming-Yuan Huang, Qin Wang, Hua-Wen Li, Yu-Guo Liu, Tang-Bin Zou
      
 PP. 95 - 106
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ABSTRACT: Naringenin is the main active component in peach leaves and has been used for prevention of several diseases, including colitis, atherosclerosis and metabolic dysregulation. In the present study, naringenin was extracted from peach (Prunus persica) leaves by ultrasonic-assisted extraction. Several important parameters such as ethanol concentration, solid-to-liquid ratio, extraction temperature and extraction time were optimized by single-factor experiment and response surface methodology. The optimal conditions were 52.2% ethanol, the liquid-to-solid ratio was 30:1, and extraction for 18.3 min at 62.0 °C under ultrasound irradiation of 200 W. Under these conditions, the yield of naringenin reached 66.17 mg/g, significantly higher than that of conventional extraction. The results obtained are very favorable for the full utilization of peach leaves, and also indicate that ultrasonic-assisted extraction is a convenient method for extracting naringenin from plant materials.


In vitro Evaluation of Some Plant Extracts, Fungal Antagonists and Organic Amendments against Fusarium oxysporum f. sp. gladioli Causing Gladiolus Wilt
Omer Jan, S. A. Ganie, V.R.Pant, M. Y. Ghani, Ashiq Hussain Lone, S.M. Razvi and Qaisar Anjum
      
 PP. 107 - 117
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ABSTRACT: Two fungal antagonists viz., Trichoderma harzianum Rifai and Trichoderma viride Pers. , two organic amendments viz. mustard cake and linseed cake and five plant extracts viz., Lantana camara, Eucalyptus globulus, Ocimum sanctum, Calotropis gigantea and Azadirachta indica were evaluated in vitro against Fusarium oxysporum f. sp. gladioli the causal pathogen of gladiolus wilt. Among fungal antagonists comparatively T. viride proved to be superior (69.49%) for inhibiting radial mycelial growth inhibition followed by T. harzianum (60.90%). Among organic amendments linseed cake (55.45%) significantly inhibited maximum mycelial growth followed by mustard cake (48.35%) as compared to control. Among plant extracts maximum mycelial growth inhibition was recorded in Azadirachta indica (43.90%), followed by Ocimum sanctum (40.79%).