International Journal of Modern Biology and Medicine
ISSN: 2165-0136 (online)Search Article(s) by:
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Table of Content for Vol. 6 No. 2, 2015

Response Surface Methodology for Ultrasound-Assisted Extraction of Naringenin from Peach (Prunus persica) Leaves
Ming-Yuan Huang, Qin Wang, Hua-Wen Li, Yu-Guo Liu, Tang-Bin Zou
      
 PP. 95 - 106
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ABSTRACT: Naringenin is the main active component in peach leaves and has been used for prevention of several diseases, including colitis, atherosclerosis and metabolic dysregulation. In the present study, naringenin was extracted from peach (Prunus persica) leaves by ultrasonic-assisted extraction. Several important parameters such as ethanol concentration, solid-to-liquid ratio, extraction temperature and extraction time were optimized by single-factor experiment and response surface methodology. The optimal conditions were 52.2% ethanol, the liquid-to-solid ratio was 30:1, and extraction for 18.3 min at 62.0 °C under ultrasound irradiation of 200 W. Under these conditions, the yield of naringenin reached 66.17 mg/g, significantly higher than that of conventional extraction. The results obtained are very favorable for the full utilization of peach leaves, and also indicate that ultrasonic-assisted extraction is a convenient method for extracting naringenin from plant materials.


In vitro Evaluation of Some Plant Extracts, Fungal Antagonists and Organic Amendments against Fusarium oxysporum f. sp. gladioli Causing Gladiolus Wilt
Omer Jan, S. A. Ganie, V.R.Pant, M. Y. Ghani, Ashiq Hussain Lone, S.M. Razvi and Qaisar Anjum
      
 PP. 107 - 117
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ABSTRACT: Two fungal antagonists viz., Trichoderma harzianum Rifai and Trichoderma viride Pers. , two organic amendments viz. mustard cake and linseed cake and five plant extracts viz., Lantana camara, Eucalyptus globulus, Ocimum sanctum, Calotropis gigantea and Azadirachta indica were evaluated in vitro against Fusarium oxysporum f. sp. gladioli the causal pathogen of gladiolus wilt. Among fungal antagonists comparatively T. viride proved to be superior (69.49%) for inhibiting radial mycelial growth inhibition followed by T. harzianum (60.90%). Among organic amendments linseed cake (55.45%) significantly inhibited maximum mycelial growth followed by mustard cake (48.35%) as compared to control. Among plant extracts maximum mycelial growth inhibition was recorded in Azadirachta indica (43.90%), followed by Ocimum sanctum (40.79%).


Comparative Analysis: ELISA and GICA for Detecting HIV Antibody among Different Dilutions
Li Fu, Xin-Bin Chen, Ze-Qian Li
      
 PP. 118 - 128
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ABSTRACT: Objective: To study and compare the sensitivity of enzyme-Linked immune sorbent assay (ELISA) and colloidal gold immune chromatographic assay (GICA) on detecting HIV antibodies. Methods: Ten HIV-positive blood samples were selected randomly. One part of each sample was made into a series of serum panel using dilution, and the other was diluted to diversified concentration. These serum panels and serial diluted samples were tested by ELISA and GIGA methods, respectively. The sensitivity of ELISA and GIGA was analyzed and compared. Results: As far as the serial serum panels examined, the sensibility of ELISA was one or two tenfold higher than that of GICA. When the samples were diluted to ten thousand times, 7 of them showed positive results by using ELISA, while 1 and 2 showed positive by using two different GICA reagents, respectively. The minimum OD value of positive reaction related to the two methods has nothing to do with HIV specific bands number. ELISA diluted multiple was usually higher than that of GICA when the samples showed the minimum positive reaction. ELISA manifested more sensitivity than GICA in detecting HIV. Conclusions: The sensitivity of ELSIA is higher than that of GICA. After taking the economic cost into consideration, as well as the practical situation of HIV testing in grassroots health institution of China mainland, GICA could be used to screen HIV for general population, but for GICA-positive blood samples, it is suggested to be re-checked by ELISA method.


Evaluation of Phytochemicals Analysis, Medicinal Properties and Nutritional Value of Sesame Seeds (Sesamum indicum. L)
Kaliyamoorthy Jayakumar, T.M. Sathees kannan, P. Thamizhiniyan and P.Vijayarengan
      
 PP. 129 - 135
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ABSTRACT: The present investigation was carried to find out the evaluation of Phytochemicals analysis, medicinal properties and nutritional value of sesame seeds (Sesamum indicum.L). Phytochemicals such as, alkaloids, saponins, tannins, glycosides and flavonoids are present in sesame seeds. The mineral content and phytochemical of sesame seeds cure various diseases like, diabetes, anemia cardiovascular health, anti-cancer, digestive health, rheumatoid arthritis, respiratory health, protection from radiation damage, bone health, oral health, anxiety and lower cholesterol.


Sugarcane Bagasse Hydrolysate-based Growth and Production of Carotenoids by Yeast Phaffia rhodozyma
Ling-Yan Zhou, Ming-Jun Zhu
      
 PP. 135 - 146
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ABSTRACT: This study evaluates the industrial waste sugarcane bagasse hydrolysate (SCBH) as a carbon source for cell growth and carotenoid production of yeast Phaffia rhodozyma. Glucose and xylose could differentially support the cell growth and carotenoid biosynthesis. Glucose is more suitable for Phaffia rhodozyma growth coupled with organic nitrogen source, as indicated by the relatively high cell dry density (ca. 10.42 g/L) and higher specific growth rate (ca. 0.24 h-1). Meanwhile, the cells cultured with glucose accumulated higher amounts of carotenoids (ca. 7.58 mg/L). Sugarcane bagasse hydrolysate as carbon source coupled with organic nitrogen source resulted in higher biomass (ca. 12.3 g/L) than glucose as carbon source and mixed carbon source (ca. glucose: xylose = 2.5). Carotenoid production of 6.10 mg/L with SCBH was almost the same as mixed carbon source. The present study suggests that SCBH is a suitable carbon source for Phaffia rhodozyma to grow and accumulate natural carotenoids.